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Image Search Results
Journal: Cell Research
Article Title: Conversion of hepatoma cells to hepatocyte-like cells by defined hepatocyte nuclear factors
doi: 10.1038/s41422-018-0111-x
Figure Lengend Snippet: Transplanted rHeps reconstructed the hepatic plate and liver lobules. a Respective HCCLM3-derived rHeps and control cells (1.5 × 107) were transplanted into the livers of Fah−/−Rag2−/− mice through spleen injection. Scale bars, 100 µm. b H&E and immunohistochemical staining of human Fah, ALB and AAT in the livers of rHep recipient Fah−/−Rag2−/− mice. c Human ALB levels in the sera of rHep-transplanted Fah−/−Rag2−/− (F/R) mice were measured by ELISA. The sera from normal human and F/R mice were used as positive and negative controls, respectively. d Fah staining of the repopulated rHeps in Fah−/−Rag2−/− mice. Images of different section areas were merged into one using Adobe Photoshop CS5 (Adobe Systems). e The percentage of Fah-positive rHeps in the liver of each rHep recipient mouse was calculated and shown. f The Kaplan-Meier survival curve of Fah−/−Rag2-/- mice that were transplanted with PHHs, control cells or rHep cells after NTBC removal. * indicates P < 0.05
Article Snippet:
Techniques: Derivative Assay, Injection, Immunohistochemical staining, Staining, Enzyme-linked Immunosorbent Assay